human cdna arrays unigem version 2 (ugv2)  (Incyte corporation)

Journal:

Article Title: The histone deacetylase inhibitor SAHA arrests cancer cell growth, up-regulates thioredoxin-binding protein-2, and down-regulates thioredoxin

doi: 10.1073/pnas.182372299

Figure Lengend Snippet: Induction of TBP-2 mRNA levels in transformed cells by SAHA. LNCaP prostate carcinoma and T24 bladder carcinoma cells were cultured with vehicle alone (0) or SAHA (2.5 or 7.5 μM) for the indicated times. Total RNA was extracted from the cells, and the levels of TBP-2 were determined by Northern blotting using a 1.1-kb 32P-labeled TBP-2 cDNA probe (Upper for each cell line). Blots were rehybridized with a γ-32P-labeled 18S oligonucleotide probe to indicate RNA loading and are shown (Lower for each cell line). Similar results were obtained for a total of six transformed cell lines.

Article Snippet: Poly(A) + mRNA from cells cultured with SAHA was compared with mRNA from cells cultured without SAHA, using the UniGEM human cDNA array (Incyte, St. Louis).

Techniques: Transformation Assay, Cell Culture, Northern Blot, Labeling

Journal:

Article Title: The histone deacetylase inhibitor SAHA arrests cancer cell growth, up-regulates thioredoxin-binding protein-2, and down-regulates thioredoxin

doi: 10.1073/pnas.182372299

Figure Lengend Snippet: Expression of TBP-2 in normal and tumor tissues. (a) Multiple tissue Northern blots, containing poly(A)+ RNA from the indicated tissues (CLONTECH), were hybridized with a 1.1-kb 32P-labeled TBP-2 cDNA probe (Upper). The bolts were rehybridized with a 2.0-kb probe for β-actin, as a control for loading (Lower). sk. muscle, skeletal muscle; per. bl. leuk., peripheral blood leukocytes. (b) A dot blot containing matched samples of cDNA extracted from normal human tissues and tumors (CLONTECH) was hybridized with a 1.1-kb 32P-labeled TBP-2 cDNA probe. Samples of colon and breast tumors (T) are shown, with the cDNA from the normal tissue (N) shown directly above each corresponding tumor sample.

Article Snippet: Poly(A) + mRNA from cells cultured with SAHA was compared with mRNA from cells cultured without SAHA, using the UniGEM human cDNA array (Incyte, St. Louis).

Techniques: Expressing, Northern Blot, Labeling, Control, Dot Blot

Journal:

Article Title: The histone deacetylase inhibitor SAHA arrests cancer cell growth, up-regulates thioredoxin-binding protein-2, and down-regulates thioredoxin

doi: 10.1073/pnas.182372299

Figure Lengend Snippet: Expression of TRX in transformed cells cultured with SAHA. T24 bladder carcinoma cells were cultured with vehicle alone (0) or 2.5 or 5 μM SAHA for 6, 15, or 24 h. RNA was extracted and analyzed by Northern blotting for levels of TRX, using a 500-bp 32P-labeled cDNA probe (Top). The blots were subsequently rehybridized with the 1.1-kb 32P-labeled TBP-2 cDNA probe to confirm induction of TBP-2 (Middle) and a γ-32P-labeled 18S oligonucleotide probe to indicate RNA loading (Bottom).

Article Snippet: Poly(A) + mRNA from cells cultured with SAHA was compared with mRNA from cells cultured without SAHA, using the UniGEM human cDNA array (Incyte, St. Louis).

Techniques: Expressing, Transformation Assay, Cell Culture, Northern Blot, Labeling